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Optica Publishing Group
  • Current Optics and Photonics
  • Vol. 5,
  • Issue 4,
  • pp. 351-361
  • (2021)

Enhancement of Speckle Contrast in vivo by Combining Linearly Polarized Laser Light and an Analyzer

Open Access Open Access

Abstract

Speckle imaging is capable of dynamic data acquisition at high spatiotemporal resolution, and has played a vital role in the functional study of biological specimens. The presence of various optical scatterers within the tissue causes alteration of speckle contrast. Thus structures like blood vessels can be delineated and quantified. Although laser speckle imaging is frequently used, an optimization process to ensure the maximum speckle contrast has not been available. In this respect, we here report an experimental procedure to optimize speckle contrast via applying different combinations of varying polarization of the illuminating laser light and multiple analyzer angles. Specifically, samples were illuminated by the p-polarization, 45°-polarization, and s-polarization of the incident laser, and speckle images were recorded without and with the analyzer rotated from 0° to 180° (Δ = 30°). Following the baseline imaging of a solid diffuser and a fixed brain sample, laser speckle contrast imaging (LSCI) was successfully performed to visualize in vivo mouse-brain blood flow. For oblique laser illumination, the maximum contrast achieved with p-polarized and s-polarized light was perpendicular to the analyzer’s axis. This study demonstrates the optimization process for maximizing the speckle contrast, which can improve blood-flow estimation in vivo.

© 2021 Optical Society of Korea

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