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Optica Publishing Group
  • Journal of the Optical Society of Korea
  • Vol. 19,
  • Issue 4,
  • pp. 382-389
  • (2015)

Motility Contrast Imaging for Drug Screening Applications

Open Access Open Access

Abstract

Motility contrast imaging is a coherence-domain imaging technique that uses cellular motility as a fully endogenous imaging contrast agent. Motility is measured inside tissue using a digital holographic coherence gate that extracts dynamic speckle from fixed depths. The dynamic speckle arises from the normal organelle motion inside cells, and from the movement of the cellular membranes driven by the cytoskeleton. It measures cellular activity and the effects of temperature and osmolarity. Motion is sensitive to cytoskeletal drugs, such as the antimitotic drugs used for cancer chemotherapy, and the effects of drug combinations also can be monitored. Motility contrast imaging is a potential tissue-based assay platform for highthroughput screening of pharmaceuticals.

© 2015 Optical Society of Korea

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