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Optica Publishing Group
  • Journal of the Optical Society of Korea
  • Vol. 20,
  • Issue 3,
  • pp. 431-434
  • (2016)

High-precision THz Dielectric Spectroscopy of Tris-HCl Buffer

Open Access Open Access

Abstract

Tris-HCl buffer solution is extensively used in biochemistry and molecular biology to maintain a stable pH for biomolecules such as nucleic acids and proteins. Here we report on the high-precision THz dielectric spectroscopy of a 10 mM Tris-HCl buffer. Using a double Debye model, including conductivity of ionic species, we measured the complex dielectric functions of Tris-HCl buffer. The fast relaxation time of water molecules in Tris-HCl buffer is ~20% longer than that in pure water while the slow relaxation time changes little. This means that the reorientation dynamics of Tris-HCl buffer with such a low Tris concentration is quite different from that of pure water.

© 2016 Optical Society of Korea

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