Abstract
Limited depth of focus (DOF) is one main shortage
in traditional optical microscopy systems that severely affect simultaneous
visualization of objects at different depths in the same field of
view. In this paper, we propose a novel method to enhance the DOF
in digital holography that consists of applying a numerical axicon
transformation to the hologram during the reconstruction process.
The idea behind this is to exploit the well-known ability of an axicon
lens to create long and narrow focal lines along the optical axis.
By this approach, we demonstrate that it is possible to obtain an
extended focused image in which objects located at different depths
are simultaneously visualized in good focus. First, the proposed method
is tested in a case study of three different wires, positioned on
different planes and recorded in lensless configuration. A comparison
with a common DOF extension approach based on cubic phase function
is performed. Finally, experiments of motile cells, flowing in a microfluidic
channel and at different depths, are investigated for demonstrating
the effectiveness of the proposed approach in bio-microfluidics.
© 2015 IEEE
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