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Infrared Spectroscopy in Bacteriological Research

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Abstract

The possible uses of the complex mixtures of compounds found in viruses (the tobacco mosaic virus) or in bacteria (the tubercle bacillus or extracts of these organisms), have been studied. It was found that the procedures of inoculation, growth, harvesting, and fractionation into simpler mixtures should be standardized. This resulted in characteristic composite spectra for each fraction. The following uses have been established for infrared spectroscopy in this field: (1) A control on the techniques of operation; (2) the discovery of the spectroscopic differences between strains which may or may not be recognized as biologically different; for example, spectroscopic differences between virulent and avirulent strains of the standard laboratory strain H37, or spectral differences between human and bovine varieties of tubercle bacilli and spectral differences between strains recently isolated from patients ill with pulmonary tuberculosis; (3) spectroscopic interpretation of molecular content.

© 1953 Optical Society of America

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