Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Design and Performance of an Infrared Microscope Attachment

Not Accessible

Your library or personal account may give you access

Abstract

An infrared microscope employing reflecting optics has been designed and constructed as an attachment to Perkin-Elmer single beam spectrometers. It is mounted after the exit slit of the monochromator in the dispersed beam to minimize possible heating and photochemical effects in the sample. The normal macro functions of the spectrometer are not disrupted by the addition of the microscope. Convenient macro-micro conversion is provided.

The condenser and objective pair, which are of the Schwarzschild type, operate at a numerical aperture of 0.75 with a 0.4 obscuration ratio and are designed to provide optimum imagery in the infrared region of the spectrum. The condenser forms an image of the exit slit at the sample space reduced 8.5×, and the objective provides a 25× enlarged image of the sample at an adjustable diaphragm. A viewing and manipulating system is provided to allow accurate positioning of small samples. The maximum field size is 0.650×0.220 mm. About 35 percent of the radiation available from the monochromator is conserved by minimizing the number of reflecting surfaces and by utilizing field mirrors to provide efficient energy transfer. The radiation is brought to a separate detector and preamplifier which are connected to the amplifier and recorder of the spectrometer.

The minimum sample size which can be effectively studied depends on wavelength and is primarily limited by the available energy. Minimum sample size and spectral dilution are discussed.

The infrared absorption spectra of fibers, crystals, and biological tissue sections, as well as solutions in cells, of extremely small volume, have been obtained using this instrument. Performance data are given and a figure of merit determined.

© 1953 Optical Society of America

Full Article  |  PDF Article
More Like This
A Reflecting Microscope for Infrared Spectrometry*

A. R. H. Cole and R. Norman Jones
J. Opt. Soc. Am. 42(5) 348-352 (1952)

Infrared Spectra of Coal and Carbohydrate Chars

R. A. Friedel and M. G. Pelipetz
J. Opt. Soc. Am. 43(11) 1051-1052 (1953)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved