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Calibration of Spectrofluorimeters for Measuring Corrected Emission Spectra

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Abstract

A rhodamine B quantum counter and a thermopile were used to calibrate a grating monochromator and photomultiplier for the measurement of corrected emission spectra from 240 to 600 mμ. The calibration was checked over a limited wavelength range with a standard tungsten lamp. Fluorescence spectra determined with the instrument agree well with previously reported spectra.

© 1962 Optical Society of America

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