Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Three-dimensional transfer functions in 4Pi confocal microscopes

Not Accessible

Your library or personal account may give you access

Abstract

We investigate three-dimensional transfer functions in order to understand image formation in the recently proposed 4Pi confocal microscope [ J. Opt. Soc. Am. A 9, 2159 ( 1992); Opt. Commun. 93, 277 ( 1992)]. Both nonfluorescence and fluorescence imaging methods are considered, and the effects of apodization functions are taken into account. It is shown that for a 4Pi confocal system of finite-sized aperture the cutoff spatial frequency in the axial direction is larger than that in the transverse direction; this behavior is opposite that of conventional confocal systems, explaining the observation of high axial resolution in 4Pi confocal systems. In addition, the three-dimensional optical transfer function for a conventional confocal fluorescence microscope of high aperture is presented.

© 1994 Optical Society of America

Full Article  |  PDF Article
More Like This
Three-dimensional coherent transfer functions in fiber-optical confocal scanning microscopes

Min Gu, X. Gan, and C. J. R. Sheppard
J. Opt. Soc. Am. A 8(7) 1019-1025 (1991)

Optical transfer functions of 4Pi confocal microscopes: theory and experiment

M. Schrader, M. Kozubek, S. W. Hell, and T. Wilson
Opt. Lett. 22(7) 436-438 (1997)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (22)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved