Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Rotational-diversity phase estimation from differential-interference-contrast microscopy images

Not Accessible

Your library or personal account may give you access

Abstract

An iterative phase-estimation method for the calculation of a specimen’s phase function or optical-path-length (OPL) distribution from differential-interference-contrast (DIC) microscopy images is presented. The method minimizes the least-squares discrepancy measure by use of the conjugate-gradient technique to estimate the phase function from multiple DIC images acquired at different specimen rotations. The estimate is regularized with a quadratic smoothness penalty. Results from testing the method with simulations and measured DIC images show improvement in the estimated phase when at least two rotationally diverse DIC images instead of a single DIC image are used for the estimation. The OPL of a cell that is estimated from two DIC images was found to be much more reliable than the OPL computed from single DIC images (which had a coefficient of variation equal to 15.8%).

© 2000 Optical Society of America

Full Article  |  PDF Article
More Like This
Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy

Chrysanthe Preza, Donald L. Snyder, and José-Angel Conchello
J. Opt. Soc. Am. A 16(9) 2185-2199 (1999)

Discrete Laplacian deconvolution for differential interference contrast microscopy

David Hammond, Scott Breitenstein, and Scott Prahl
J. Opt. Soc. Am. A 39(1) 53-62 (2022)

Quantitative Carré differential interference contrast microscopy to assess phase and amplitude

Donald D. Duncan, David G. Fischer, Amanda Dayton, and Scott A. Prahl
J. Opt. Soc. Am. A 28(6) 1297-1306 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (24)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved