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Macular pigment density assessed by directional fundus reflectance

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Abstract

Light radiated from foveal photoreceptors was analyzed in the eye’s pupil at 470nm and 532nm. The reflectance of the inner limiting membrane was then measured at 6deg from the fovea for the same wavelengths, allowing us to determine the macular pigment (MP) density Ddir using the directional reflectance technique. In addition we measured the MP density Dnd using the nondirectional reflectance technique (26 subjects). The mean values of Ddir and Dnd were 0.419±0.097 and 0.195±0.042D.U., respectively (sample field of 2deg). They were highly correlated (p<0.0001). Comparison of Ddir and Dnd implies that 57±12% of the light reflected from the fovea comes from layers anterior to MP at 470nm. The mean directionality factors ρ that we have measured at 470nm and 532nm were equal to 0.239±0.028 and 0.210±0.028mm2, respectively. They were correlated (p<0.0001) and followed the spectral dependence suggested by Marcos.

© 2009 Optical Society of America

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