Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Optimized extended depth of focus simulated analysis for confocal microscopy

Not Accessible

Your library or personal account may give you access

Abstract

In imaging systems and especially in confocal microscopy systems there is a trade-off between the lateral resolution and the obtained depth of focus. The use of complex pupils to improve the lateral resolution by engineering the point spread function is a common approach; however the lateral improvement reduces the effective depth of focus and therefore the fluorescence efficiency. In this work we analytically develop an optimized approach for obtaining a complex pupil with an extended depth of focus. The proposed solution is numerically applied and tested in designing an improved focal depth in confocal microscope configuration.

© 2010 Optical Society of America

Full Article  |  PDF Article
More Like This
Optimized circularly symmetric phase mask to extend the depth of focus

Feng Zhou, Ran Ye, Guangwei Li, Haitao Zhang, and Dongsheng Wang
J. Opt. Soc. Am. A 26(8) 1889-1895 (2009)

Scanning holographic microscopy with transverse resolution exceeding the Rayleigh limit and extended depth of focus

Guy Indebetouw, Alouahab El Maghnouji, and Richard Foster
J. Opt. Soc. Am. A 22(5) 892-898 (2005)

Extended depth of focus multiphoton microscopy via incoherent pulse splitting

Bingying Chen, Tonmoy Chakraborty, Stephan Daetwyler, James D. Manton, Kevin Dean, and Reto Fiolka
Biomed. Opt. Express 11(7) 3830-3842 (2020)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (25)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved