Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Fast and accurate three-dimensional point spread function computation for fluorescence microscopy

Not Accessible

Your library or personal account may give you access

Abstract

The point spread function (PSF) plays a fundamental role in fluorescence microscopy. A realistic and accurately calculated PSF model can significantly improve the performance in 3D deconvolution microscopy and also the localization accuracy in single-molecule microscopy. In this work, we propose a fast and accurate approximation of the Gibson–Lanni model, which has been shown to represent the PSF suitably under a variety of imaging conditions. We express the Kirchhoff’s integral in this model as a linear combination of rescaled Bessel functions, thus providing an integral-free way for the calculation. The explicit approximation error in terms of parameters is given numerically. Experiments demonstrate that the proposed approach results in a significantly smaller computational time compared with current state-of-the-art techniques to achieve the same accuracy. This approach can also be extended to other microscopy PSF models.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
On-the-fly estimation of a microscopy point spread function

Jizhou Li, Feng Xue, Fuyang Qu, Yi-Ping Ho, and Thierry Blu
Opt. Express 26(20) 26120-26133 (2018)

Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments

Elie Maalouf, Bruno Colicchio, and Alain Dieterlen
J. Opt. Soc. Am. A 28(9) 1864-1870 (2011)

Single shot, three-dimensional fluorescence microscopy with a spatially rotating point spread function

Zhaojun Wang, Yanan Cai, Yansheng Liang, Xing Zhou, Shaohui Yan, Dan Dan, Piero R. Bianco, Ming Lei, and Baoli Yao
Biomed. Opt. Express 8(12) 5493-5506 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (14)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved