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Versatile transmission/reflection tomographic diffractive microscopy approach

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Abstract

Tomographic diffractive microscopy (TDM) has gained interest in recent years due to its ability to deliver high-resolution, three-dimensional images of unlabeled samples. It has been applied to transparent samples in transmission mode, as well as to surface studies in reflection mode. Mudry et al. [Opt. Lett. 35, 1857 (2010) [CrossRef]  ] introduced the concept of mirror-assisted TDM (MA-TDM), an elegant approach for achieving quasi-isotropic-resolution microscopic imaging, but which is still to be experimentally applied. In this work, we show that a simplified version of MA-TDM allows for transforming a reflective TDM setup into a more versatile instrument, also capable of observing transparent samples in transmission mode if using specific sample holders made out of a mirror and coated with a low-thickness transparent spacer.

© 2019 Optical Society of America

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Supplementary Material (1)

NameDescription
Visualization 1       Mirroring effect in TDM, observed with high incidence illumination.

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