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Method for virtual optical sectioning and tomography utilizing shallow depth of field

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Abstract

A method is proposed for high-resolution, three-dimensional reconstruction of internal structures of objects from planar transmission images. The described approach can be used with any form of radiation or matter waves, in principle, provided that the depth of field is smaller than the thickness of the sample. The physical optics basis for the method is elucidated, and the reconstruction algorithm is presented in detail. A simulated example demonstrates an application of the method to three-dimensional electron transmission imaging of a nanoparticle under realistic radiation dose and spatial resolution constraints. It is envisaged that the method can be applicable in high-resolution transmission electron microscopy, soft x-ray microscopy, ultrasound imaging, and other areas.

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Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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