Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Optical microscope tomography. I. Support constraint

Not Accessible

Your library or personal account may give you access

Abstract

A new principle of tomographic optical microscope imaging has been developed that uses a computerized reconstruction algorithm and a transmission optical microscope. A conventional transmission microscope with a rotationally oblique illumination system provides projections of a thick specimen in various directions within the numerical aperture of the objective lens. The images obtained are combined to reconstruct a three-dimensional distribution of the sample by inverting the imaging system in a computer. The three-dimensional optical transfer function of this projection system is analyzed, and it is found that this system is strictly angularly band limited. For improving the spatial resolution we utilize a priori knowledge of the spatial extent of the object as the support constraint. Experimental results are presented to demonstrate the tomographic imaging capability of this principle.

© 1987 Optical Society of America

Full Article  |  PDF Article
More Like This
Optical microscope tomography. II. Nonnegative constraint by a gradient-projection method

O. Nakamura, S. Kawata, and S. Minami
J. Opt. Soc. Am. A 5(4) 554-561 (1988)

Phase retrieval for discrete functions with support constraints

Thomas R. Crimmins
J. Opt. Soc. Am. A 4(1) 124-134 (1987)

Three-dimensional transfer-function analysis of the tomographic capability of a confocal fluorescence microscope

Osamu Nakamura and Satoshi Kawata
J. Opt. Soc. Am. A 7(3) 522-526 (1990)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (12)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved