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Image enhancement and field of view enlargement in digital lensless holographic microscopy by multi-shot imaging

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Abstract

A method to improve the quality of reconstructed images while the field of view (FOV) is enlarged in digital lensless holographic microscopy (DLHM) is presented. Multiple DLHM holograms are recorded while a still sample is located at different places of the plane containing it. The different locations of the sample must produce a set of DLHM holograms that share an overlapped area with a fixed DLHM hologram. The relative displacement among multiple DLHM holograms is computed by means of a normalized cross-correlation. The value of the computed displacement is utilized to produce a new DLHM hologram resulting from the coordinated addition of multi-shot DLHM holograms with the corresponding compensated displacement. The composed DLHM hologram carries enhanced information of the sample in a larger format, leading to a reconstructed image with improved quality and larger FOV. The feasibility of the method is illustrated and validated with results obtained from imaging a calibration test target and a biological specimen.

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Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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