Abstract

The transport of intensity equation (TIE) allows to recover the phase of a microscopy sample from differently focused intensity measures along the axial direction of its optical field. In the present work, we propose a cost-effective technique for snapshot phase retrieval with TIE. The optics of a commercially available camera is replaced with a doublet system consisting of a microscope objective and a lenslet array with an extra lens mask attached to it. The system allows to obtain, in real-time and with no mechanical shift of either the sample or the sensor, the in-focus as well as a defocused image of the sample. From these two sub-aperture images, the intensity derivative term in TIE can then be approximated after image rectification. Phase is then retrieved for static as well as dynamic samples over the common view area. Validation experiments are presented.

© 2024 Optica Publishing Group

Transport of intensity and phase: applications to digital holography [Invited]

Nouf A. Alanazi, Austin M. Scott, Hammid Al-Ghezi, Muhammad Faryad, Akhlesh Lakhtakia, and Partha P. Banerjee
Appl. Opt. 63(10) 2436-2454 (2024)

Optical module for single-shot quantitative phase imaging based on the transport of intensity equation with field of view multiplexing

Jose Angel Picazo-Bueno and Vicente Micó
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Optical convolution for quantitative phase retrieval using the transport of intensity equation

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Appl. Opt. 57(1) A134-A141 (2018)

Non-recursive transport of intensity phase retrieval with the transport of phase

Haowen Zhou, Haiyun Guo, and Partha P. Banerjee
Appl. Opt. 61(5) B190-B199 (2022)

Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods

Jeremy M. Wittkopp, Ting Chean Khoo, Shane Carney, Kai Pisila, Shahab J. Bahreini, Kate Tubbesing, Supriya Mahajan, Anna Sharikova, Jonathan C. Petruccelli, and Alexander Khmaladze
Opt. Express 28(5) 6123-6133 (2020)