Abstract

Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, ${{\rm i}^2}{\rm PIE}$, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, ${{\rm i}^2}{\rm PIE}$ leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.

© 2020 Optical Society of America

Implementation of temporal ptychography algorithm, i²PIE, for improved single-beam coherent anti-Stokes Raman scattering measurements

Ruan Viljoen, Pieter Neethling, Dirk Spangenberg, Alexander Heidt, Hans-Martin Frey, Thomas Feurer, and Erich Rohwer
J. Opt. Soc. Am. B 37(11) A259-A265 (2020)

Temporal fine structure of all-normal dispersion fiber supercontinuum pulses caused by non-ideal pump pulse shapes

Anupamaa Rampur, Dirk-Mathys Spangenberg, Grzegorz Stępniewski, Dominik Dobrakowski, Karol Tarnowski, Karolina Stefańska, Adam Paździor, Paweł Mergo, Tadeusz Martynkien, Thomas Feurer, Mariusz Klimczak, and Alexander M. Heidt
Opt. Express 28(11) 16579-16593 (2020)

Decoherence due to XPM-assisted Raman amplification for polarization or wavelength offset pulses in all-normal dispersion supercontinuum generation

James S. Feehan and Jonathan H. V. Price
J. Opt. Soc. Am. B 37(3) 635-644 (2020)

Tailoring the waveguide dispersion of nonlinear fibers for supercontinuum generation with superior intrapulse coherence

Chao Mei and Günter Steinmeyer
J. Opt. Soc. Am. B 37(8) 2485-2497 (2020)

Actively shaped supercontinuum from a photonic crystal fiber for nonlinear coherent microspectroscopy

Bernhard von Vacano, Wendel Wohlleben, and Marcus Motzkus
Opt. Lett. 31(3) 413-415 (2006)