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Video-rate three-dimensional optical coherence tomography

Markus Laubscher, Mathieu Ducros, Boris Karamata, Theo Lasser, and René Salathé

Open Access Open Access

Abstract

Most current optical coherence tomography systems provide two-dimensional cross-sectional or en face images. Successive adjacent images have to be acquired to reconstruct three-dimensional objects, which can be time consuming. Here we demonstrate three-dimensional optical coherence tomography (3D OCT) at video rate. A 58 by 58 smart-pixel detector array was employed. A sample volume of 210x210x80 μm3 (corresponding to 58x58x58 voxels) was imaged at 25 Hz. The longitudinal and transverse resolutions are 3 μm and 9 μm respectively. The sensitivity of the system was 76 dB. Video rate 3D OCT is illustrated by movies of a strand of hair undergoing fast thermal damage.

©2002 Optical Society of America

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Supplementary Material (2)

Media 1: MOV (149 KB)     
Media 2: MOV (740 KB)     

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Figures (4)
Fig. 1.
Fig. 1. Parallel OCT optical setup schematic. The different elements are: mode-locked Ti:Sapphire femtosecond laser (MLTS); achromatic lenses (L1, L2, L3, and L6); non-polarizing achromatic beamsplitter cube (BS); identical achromatic microscope objectives 20X, 0.45 NA (L4 and L5); reference mirror (RM); variable neutral density filter wheel (F); compensation glass plate (C); 58 by 58 smart pixel detector array (SPDA) and sample (S).

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Fig. 2.
Fig. 2. (left) Schematic of the imaged volume (dashed parallelepiped) in relation to the sample (hair strand on glass slide). (center) En face image (210x210 μm2) at the height of the contact between hair and glass. (right top) Longitudinal cut (210x80 μm2) parallel to and at the center of the hair. (right bottom) Cross-sectional cut (210x80 μm2) perpendicular to the axis of the hair strand.

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Fig. 3.
Fig. 3. (149 kB) Tomographic images acquired during a 1600ms time interval at a rate of 25 volumes per second (40 time frames). (left) En face image (210x210 μm2) at the height of the contact between hair and glass. (center) Cross-sectional cut (210x80 μm2). (right) Longitudinal cut (210x80 μm2) parallel to and at the center of the hair. Reflectivity grayscale as in Figure 2.

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Fig.4.
Fig.4. (740 kB) Movie of a 3D rendering of the sample based on isosurfaces. To facilitate the comprehension of this particular perspective we indicate the situation of the hair and the glass slide by the colored lines in the first frame.

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Equations (1)

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S = 20 log V 2 % max σ + 10 log 1 0.02
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