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Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars

Robert W. Applegate, Jeff Squier, Tor Vestad, John Oakey, and David W. M. Marr

Open Access Open Access

Abstract

We demonstrate a new technique for trapping, sorting, and manipulating cells and micrometer-sized particles within microfluidic systems, using a diode laser bar. This approach overcomes the scaling limitations of conventional scanned laser traps, while avoiding the computational and optical complexity inherent to holographic optical trapping schemes. The diode laser bar enables us to control a large trapping zone, 1 μm by 100 μm, without the necessity of scanning or altering the phase of the beam.

©2004 Optical Society of America

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Supplementary Material (6)

Media 1: MOV (99 KB)     
Media 2: MOV (839 KB)     
Media 3: MOV (80 KB)     
Media 4: MOV (94 KB)     
Media 5: MOV (493 KB)     
Media 6: MOV (217 KB)     

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Figures (13)
Fig. 1.
Fig. 1. Schematic of diode laser bar trapping and imaging system

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Fig. 2.
Fig. 2. Single lines of trapped 1.8 μm colloids, 10 μm colloids, and bovine red blood cells

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Fig. 3.
Fig. 3. Drawing of the razorblade mask

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Fig. 4.
Fig. 4. A montage of a single 1.8 μm polystyrene colloid manually translated the length of the trap

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Fig. 5.
Fig. 5. A montage of a single 10 μm polystyrene colloid manually translated the length of the trap

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Fig. 6.
Fig. 6. A montage of a single bovine red blood cell manually translated the length of the trap

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Fig. 7.
Fig. 7. (104 Kb) A movie of 9 μm particles flowing into the trap line and being diverted (line shows laser location)

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Fig. 8.
Fig. 8. (844 Kb) A 9 μm colloid being moved the length of the trap within a flowing microfluidic channel (line shows laser location)

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Fig. 9.
Fig. 9. (84 Kb) A movie of bovine red blood cells flowing into the trap line and being diverted along the length of the trap (line shows laser location)

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Fig. 10.
Fig. 10. (100 Kb) A movie of bovine blood cells being allowed or disallowed in a region of the flowing channel (line shows laser location)

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Fig. 11.
Fig. 11. A numeric simulation the streamlines and relative flow rates within the 7 channel sorting system

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Fig. 12.
Fig. 12. (500 Kb) A movie of multiple bovine red blood cells. The first is trapped and sent into a different channel. The second is allowed to pass through (line shows laser location).

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Fig. 13.
Fig. 13. (224 Kb) Bovine blood cells directed to different parts of the channel (line shows laser location)

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