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Zeeman laser-scanning confocal microscopy in turbid media

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Abstract

A novel Zeeman laser-scanning confocal microscope (ZLSCM) is proposed. It has the same configuration as the conventional laser-scanning confocal microscope (LSCM) in which a Zeeman laser in conjunction with a Glan–Thompson analyzer is used. In our system, the analyzer with the bandpass filter, which act simultaneously as a polarization gate and a coherence gate, enhance the collection efficiency of the weak-scattering photons and simultaneously suppress the multiple-scattering photons. The improvement in depth resolution of a ZLSCM in a scattering medium compared with that of a conventional LSCM is discussed and demonstrated experimentally.

© 2001 Optical Society of America

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