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Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging

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Abstract

We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40 µm×60 µm×3 µm. With the technique presented, the structure–mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.

© 2003 Optical Society of America

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