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Spectrally encoded slit confocal microscopy

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Abstract

A simple and cost-effective method for real-time imaging in confocal microscopy is proposed. Spectrally encoded slit confocal microscopy (SESCoM) uses a spectral encoding technique together with a confocal slit aperture to achieve two-dimensional images. Simulation and experimental results of the SESCoM’s axial and lateral performances are presented. The measured FWHM of the axial response is 1.15μm when an objective with a NA of 0.95 is used. FWHMs of the lateral line spread functions are measured to be 236 and 244nm along the x and y directions, respectively. Both the axial and the lateral experimental results agree well with the simulation results.

© 2006 Optical Society of America

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