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Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source

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Abstract

We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of 5ps duration and having a maximum peak power of over 100 W at a wavelength of 800nm and a repetition frequency of 500MHz.

© 2007 Optical Society of America

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