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Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit

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Abstract

We report near-optimal tracking of freely diffusing fluorescent particles in a quasi-two-dimensional geometry via photon counting and real-time feedback. We present a quantitative statistical model of our feedback network and find excellent agreement with the experiment. We monitor the motion of a single fluorescent particle with a sensitivity of 15nmHz while collecting fewer than 5000fluorescencephotonss. Fluorescent microspheres (diffusion coefficient 1.3μm2s) are tracked with a root-mean-square tracking error of 170nm, within a factor of 2 of the theoretical limit set by photon counting shot noise.

© 2006 Optical Society of America

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