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Reflective objective-based ultraviolet photoacoustic remote sensing virtual histopathology

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Abstract

Histopathological examination typically involves tissue resection or biopsy, fixation, sectioning, and staining protocols. A non-contact high-resolution photoacoustic remote sensing microscopy system is presented which is capable of depth-resolved imaging of cell nuclei in fixed and fresh tissues without the need for stains or labels. The reflection-mode system is based on a 0.5 numerical aperture reflective objective and enables fast optical scanning using a 600 kHz repetition rate fiber laser to produce histological-like images with 0.39 µm resolution and with close agreement to traditional H&E and fluorescence staining.

© 2020 Optical Society of America

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