Abstract

The subtraction imaging in confocal microscopy is demonstrated by using two vector beams with radial and azimuthal polarizations. The longitudinal electric component, which appears near the focus of a radially polarized beam under the tight focusing condition and produces a smaller focal spot, is effectively extracted by the subtraction using an azimuthally polarized beam. This subtraction imaging with vector beams provides the improvement of the lateral resolution in confocal microscopy without the degradation due to the excess subtraction.

© 2014 Optical Society of America

Resolution enhancement of confocal microscopy by subtraction method with vector beams

Susumu Segawa, Yuichi Kozawa, and Shunichi Sato
Opt. Lett. 39(11) 3118-3121 (2014)

Subtraction imaging by the combination of higher-order vector beams for enhanced spatial resolution

Mio Yoshida, Yuichi Kozawa, and Shunichi Sato
Opt. Lett. 44(4) 883-886 (2019)

Imaging with a longitudinal electric field in confocal laser scanning microscopy to enhance spatial resolution

Yuichi Kozawa, Ryota Sakashita, Yuuki Uesugi, and Shunichi Sato
Opt. Express 28(12) 18418-18430 (2020)

Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector

Emilio Sánchez-Ortiga, Colin J. R. Sheppard, Genaro Saavedra, Manuel Martínez-Corral, Ana Doblas, and Arnau Calatayud
Opt. Lett. 37(7) 1280-1282 (2012)

Confocal supercritical angle microscopy for cell membrane imaging

Siddharth Sivankutty, Thomas Barroca, Céline Mayet, Guillaume Dupuis, Emmanuel Fort, and Sandrine Lévêque-Fort
Opt. Lett. 39(3) 555-558 (2014)