Abstract
Optical projection tomography (OPT) is a powerful tool for three-dimensional (3D) imaging of mesoscopic samples. While it is able to achieve resolution of a few tens of microns over a sample volume of several cubic centimeters, the reconstructed images often suffer from artifacts caused by inaccurate calibration. In this work, we focus on the refractive-index mismatch between the sample and the surrounding medium. We derive a 3D cone-beam forward model of OPT that approximates the effect of refractive-index mismatch. We then implement a fast and efficient reconstruction method to correct for the induced seagull-shaped artifacts on experimental images of fluorescent beads.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Yan Liu, Jonathan Dong, Thanh-an Pham, François Marelli, and Michael Unser
Opt. Continuum 1(12) 2577-2589 (2022)
Yunhui Gao and Liangcai Cao
Opt. Lett. 47(22) 5965-5968 (2022)
Samuel P. X. Davis, Laura Wisniewski, Sunil Kumar, Teresa Correia, Simon R. Arridge, Paul Frankel, James McGinty, and Paul M. W. French
Opt. Lett. 43(22) 5555-5558 (2018)