Abstract

We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.

© 1997 Optical Society of America

Optical sectioning in wide-field microscopy obtained by dynamic structured light illumination and detection based on a smart pixel detector array

Jelena Mitić, Tiemo Anhut, Matthias Meier, Mathieu Ducros, Alexander Serov, and Theo Lasser
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Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

V. Poher, H. X. Zhang, G. T. Kennedy, C. Griffin, S. Oddos, E. Gu, D. S. Elson, J. M. Girkin, P.M.W. French, M.D. Dawson, and M.A.A. Neil
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Single-exposure optical sectioning by color structured illumination microscopy

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Optical transfer functions of 4Pi confocal microscopes: theory and experiment

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Optimization and characterization of a structured illumination microscope

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Opt. Express 15(24) 16130-16140 (2007)