Abstract

We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.

© 1997 Optical Society of America

Optical sectioning in wide-field microscopy obtained by dynamic structured light illumination and detection based on a smart pixel detector array

Jelena Mitić, Tiemo Anhut, Matthias Meier, Mathieu Ducros, Alexander Serov, and Theo Lasser
Opt. Lett. 28(9) 698-700 (2003)

Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

V. Poher, H. X. Zhang, G. T. Kennedy, C. Griffin, S. Oddos, E. Gu, D. S. Elson, J. M. Girkin, P.M.W. French, M.D. Dawson, and M.A.A. Neil
Opt. Express 15(18) 11196-11206 (2007)

Optical sectioning robotic microscopy for everyone: the structured illumination microscope with the OpenFlexure stages

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Opt. Express 30(13) 23208-23216 (2022)

Single-exposure optical sectioning by color structured illumination microscopy

Leo G. Krzewina and Myung K. Kim
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Optical transfer functions of 4Pi confocal microscopes: theory and experiment

M. Schrader, M. Kozubek, S. W. Hell, and T. Wilson
Opt. Lett. 22(7) 436-438 (1997)