Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Spatial photoselection of single molecules on the surface of spherical microcavities

Not Accessible

Your library or personal account may give you access

Abstract

We show that ultrasensitive microdroplet-stream fluorescence techniques combined with surfactant forms of Rhodamine dyes can be used to probe single molecules on the surfaces of spherical microcavities. Individual octadecyl Rhodamine B molecules, shown previously by ensemble measurements to be localized and oriented at the surfaces of liquid microspheres, were spatially photoselected primarily along great circles lying perpendicular or parallel to the detection axis by use of polarized laser excitation. A polarization dependence is observed in the distribution of single-molecule fluorescence amplitudes that can be interpreted qualitatively in terms of position-dependent fluorescence-collection efficiencies.

© 1998 Optical Society of America

Full Article  |  PDF Article
More Like This
Real-time observation of single-molecule fluorescence in microdroplet streams

M. D. Barnes, N. Lermer, C.-Y. Kung, W. B. Whitten, J. M. Ramsey, and S. C. Hill
Opt. Lett. 22(16) 1265-1267 (1997)

Excitation localization principle for spherical microcavities

H.-B. Lin, J. D. Eversole, A. J. Campillo, and J. P. Barton
Opt. Lett. 23(24) 1921-1923 (1998)

Collection of fluorescence from single molecules in microspheres: effects of illumination geometry

Steven C. Hill, Michael D. Barnes, William B. Whitten, and J. Michael Ramsey
Appl. Opt. 36(19) 4425-4437 (1997)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved