Abstract

We describe and illustrate experimentally a method aimed at the three-dimensional (3D) imaging of fluorescent inhomogeneities embedded in a turbid medium. The method utilizes incoherent scanning holography to capture 3D information in a single two-dimensional scan and phase-sensitive heterodyne detection to reject multiply scattered light and to produce a single-sideband holographic record. The 3D imaging capability of the method is illustrated by an example.

© 1998 Optical Society of America

Three-dimensional holographic fluorescence microscopy

Bradley W. Schilling, Ting-Chung Poon, Guy Indebetouw, Brian Storrie, K. Shinoda, Y. Suzuki, and Ming Hsien Wu
Opt. Lett. 22(19) 1506-1508 (1997)

Direct-to-video holographic readout in quantum wells for three-dimensional imaging through turbid media

R. Jones, N. P. Barry, S. C. W. Hyde, P. M. W. French, K. W. Kwolek, D. D. Nolte, and M. R. Melloch
Opt. Lett. 23(2) 103-105 (1998)

Three-dimensional microscopy with phase-shifting digital holography

Tong Zhang and Ichirou Yamaguchi
Opt. Lett. 23(15) 1221-1223 (1998)

Twin-image elimination experiments for three-dimensional images in optical scanning holography

T.-C. Poon, T. Kim, G. Indebetouw, B. W. Schilling, M. H. Wu, K. Shinoda, and Y. Suzuki
Opt. Lett. 25(4) 215-217 (2000)

Scanning holographic microscopy of three-dimensional fluorescent specimens

Guy Indebetouw and Wenwei Zhong
J. Opt. Soc. Am. A 23(7) 1699-1707 (2006)