Abstract
A method for increasing lateral as well as axial resolution in fluorescence
microscopy is presented. A passband with a high cutoff frequency throughout
reciprocal space can be achieved by illumination of the object with spatially
harmonic excitation patterns generated by the interference of two collimated
laser beams. Theoretical calculations show an almost isotropic point-spread
function with a FWHM near 100 nm.
© 2001 Optical Society of America
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