Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Two-color two-photon 4Pi fluorescence microscopy

Not Accessible

Your library or personal account may give you access

Abstract

In 4Pi fluorescence microscopy the point-spread function is composed of a strong central lobe accompanied by interference sidelobes that produce artifacts in the image. We propose to combine two-color two-photon fluorescence microscopy and 4Pi fluorescence microscopy to overcome this sidelobe problem. Simulation results show that a single sharp fluorescence spot can be produced by use of two excitation wavelengths of 400 and 800 nm and detected at 350-nm emission wavelength.

© 2004 Optical Society of America

Full Article  |  PDF Article
More Like This
4Pi microscopy of type A with 1-photon excitation in biological fluorescence imaging

Marion Lang, Tobias Müller, Johann Engelhardt, and Stefan W. Hell
Opt. Express 15(5) 2459-2467 (2007)

4Pi confocal microscopy with alternate interference

Stefan W. Hell and Matthias Nagorni
Opt. Lett. 23(20) 1567-1569 (1998)

Polarization effects in 4Pi confocal microscopy studied with water-immersion lenses

Karsten Bahlmann and Stefan W. Hell
Appl. Opt. 39(10) 1652-1658 (2000)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (5)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved