Abstract

A simple time-domain optical method for estimating the depth and concentration of fluorescent inclusions in turbid media is described. We demonstrate direct depth estimation of a localized fluorescent object from the temporal position of the temporal point-spread function maximum. The depth estimation permits recovery of the fluorophore concentration, both of which are essential quantities for optical molecular imaging studies. Since the maximum is independent of the fluorophore concentration, excitation laser power, detector gain, and other system-dependent factors, this method ensures a robust and efficient approach.

© 2004 Optical Society of America

Estimating the depth and lifetime of a fluorescent inclusion in a turbid medium using a simple time-domain optical method

Sung-Ho Han and David J. Hall
Opt. Lett. 33(9) 1035-1037 (2008)

Analytical method for localizing a fluorescent inclusion in a turbid medium

A. Laidevant, A. Da Silva, M. Berger, J. Boutet, J.-M. Dinten, and A. C. Boccara
Appl. Opt. 46(11) 2131-2137 (2007)

Time-domain geometrical localization of point-like fluorescence inclusions in turbid media with early photon arrival times

Julien Pichette, Jorge Bouza Domínguez, and Yves Bérubé-Lauzière
Appl. Opt. 52(24) 5985-5999 (2013)

Real time optical method for localization of inclusions embedded in turbid media

Anabela Da Silva, Nadia Djaker, Nicolas Ducros, Jean-Marc Dinten, and Philippe Rizo
Opt. Express 18(8) 7753-7762 (2010)

Quantitative optical imaging of the pharmacokinetics of fluorescent-specific antibodies to tumor markers through tissuelike turbid media

Israel Gannot, Avital Garashi, Victor Chernomordik, and Amir Gandjbachkhe
Opt. Lett. 29(7) 742-744 (2004)