Abstract

We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells.

© 2004 Optical Society of America

Full Article  |  PDF Article
More Like This
Time-resolved fluorescence imaging of solvent interactions in microfluidic devices

Richard K. P. Benninger, Oliver Hofmann, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark A. A. Neil, Andrew J. deMello, and Paul M. W. French
Opt. Express 13(16) 6275-6285 (2005)

High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging

J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. H. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw
Opt. Lett. 29(19) 2249-2251 (2004)

Simultaneous FRAP, FLIM and FAIM for measurements of protein mobility and interaction in living cells

James A. Levitt, Penny E. Morton, Gilbert O. Fruhwirth, George Santis, Pei-Hua Chung, Maddy Parsons, and Klaus Suhling
Biomed. Opt. Express 6(10) 3842-3854 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics