Abstract

We present a new type of flow cytometer that can operate underwater for a long time, as long as days, for measuring the size distribution, concentration, and biomass of marine phytoplankton. The major improvement of the instrument over existing techniques is the elimination of sample preparation, which is achieved with a laser Doppler crossed-beam arrangement for both defining a measurement volume and measuring the speed of the particle traversing it. By simultaneously sampling the laser-induced fluorescence signal and the Doppler signals, the technique can discriminate sizes of phytoplankton.

© 2005 Optical Society of America

Real-time size distribution, concentration, and biomass measurement of marine phytoplankton with a novel dual-beam laser fluorescence Doppler cytometer

Robert K. Y. Chan and Ka Man Un
Appl. Opt. 40(18) 2956-2965 (2001)

A fast fluorescence imaging flow cytometer for phytoplankton analysis

Jianglai Wu and Robert K. Y. Chan
Opt. Express 21(20) 23921-23926 (2013)

Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence

Luca Fiorani, Valeri P. Maltsev, Vyacheslav M. Nekrasov, Antonio Palucci, Konstantin A. Semyanov, and Valeria Spizzichino
Appl. Opt. 47(24) 4405-4412 (2008)

RETRACTED: Simultaneous dual-color light sheet fluorescence imaging flow cytometry for high-throughput marine phytoplankton analysis

Jianping Li and Zhennan Xu
Opt. Express 25(12) 13602-13616 (2017)

Compact system for in situ laser Doppler velocimetry of blood flow

Elise Bou, Aliou Ly, Julien Roul, Olivier Llopis, Christophe Vieu, and Aline Cerf
Biomed. Opt. Express 10(11) 5862-5876 (2019)