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In situ determination of refractive index and size of Bacillus spores by light transmission

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Abstract

Light-extinction measurements in the wavelength range of 400to1000nm are performed in situ on Bacillus subtilis endospores during heat-shock-induced activation. Simultaneous information on particle size and refractive indices during activation is calculated from the transmission spectra by use of the Gaussian ray approximation of anomalous diffraction theory. During activation the refractive index of the core decreases from 1.51 to 1.39, and the size increases from 0.38to0.6μm.

© 2005 Optical Society of America

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