Abstract
A single-cell diagnostic technique for epithelial cancers is developed by utilizing laser trapping and Raman spectroscopy to differentiate cancerous and normal epithelial cells. Single-cell suspensions were prepared from surgically removed human colorectal tissues following standard primary culture protocols and examined in a near-infrared laser-trapping Raman spectroscopy system, where living epithelial cells were investigated one by one. A diagnostic model was built on the spectral data obtained from 8 patients and validated by the data from 2 new patients. Our technique has potential applications from epithelial cancer diagnosis to the study of cell dynamics of carcinogenesis.
© 2006 Optical Society of America
Full Article | PDF ArticleMore Like This
Shaoxin Li, Gong Chen, Yanjiao Zhang, Zhouyi Guo, Zhiming Liu, Junfa Xu, Xueqiang Li, and Lin Lin
Opt. Express 22(21) 25895-25908 (2014)
Duo Lin, Shangyuan Feng, Jianji Pan, Yanping Chen, Juqiang Lin, Guannan Chen, Shusen Xie, Haishan Zeng, and Rong Chen
Opt. Express 19(14) 13565-13577 (2011)
Yicong Wu and Jianan Y. Qu
Opt. Lett. 31(12) 1833-1835 (2006)