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Hyper-Raman microspectroscopy: a new approach to completing vibrational spectral and imaging information under a microscope

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Abstract

We have developed hyper-Raman scattering microspectroscopy and applied it to a microcrystal of all-trans-β-carotene. The hyper-Raman spectrum of all-trans-β-carotene exhibits a Raman-inactive but infrared-active vibrational mode at 1564cm1. Hyper-Raman imaging of a microcrystal was performed with this band. Infrared-active vibrational imaging was achieved with a spatial resolution much higher than that of conventional infrared microscopy. The combination of Raman and hyper-Raman spectroscopy opens up a new scope for high-spatial-resolution vibrational microspectroscopy that is not restricted by the selection rule.

© 2006 Optical Society of America

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