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Unified Mie and fractal scattering by biological cells and subcellular structures

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Abstract

Angle-resolved light scattering spectroscopy of biological cells is investigated in the visible wavelength range. A unified Mie and fractal model is shown to provide an accurate global agreement with light scattering spectra from 1.1° to 165° scattering angles. It is found that light scattering in forward directions (<8°) is dominated by Mie scattering by the bare cell and nucleus, whereas light scattering at large angles (>20°) is determined by fractal scattering by subcellular structures. The findings are consistent with the results of experimental investigation of the contributions of different cellular components to light scattering by cells.

© 2007 Optical Society of America

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