Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Florian Charrière; Anca Marian; Tristan Colomb; Pierre Marquet; Christian Depeursinge

doi:10.1364/OL.32.002456

Optics Letters
Vol. 32,
Issue 16,
pp. 2456-2458
(2007)
•https://doi.org/10.1364/OL.32.002456

Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Florian Charrière, Anca Marian, Tristan Colomb, Pierre Marquet, and Christian Depeursinge

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Florian Charrière, Anca Marian, Tristan Colomb, Pierre Marquet, and Christian Depeursinge, "Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy," Opt. Lett. 32, 2456-2458 (2007)

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Abstract

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a $100 \times$ 1.3 NA MO are presented.

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Polynomial	Cartesian Form	Description	Coefficient
$Z_{0}$	1	Piston	$- 4.930$
$Z_{1}$	2x	Tilt y	0.051
$Z_{2}$	2y	Tilt y	$- 0.008$
$Z_{3}$	$3^{1 ∕ 2} (2 x^{2} + 2 y^{2} - 1)$	Power	$- 0.338$
$Z_{4}$	$6^{1 ∕ 2} (2 xy)$	Astig. y	0.038
$Z_{5}$	$6^{1 ∕ 2} (x^{2} - y^{2})$	Astig. x	0.069
$Z_{6}$	$8^{1 ∕ 2} (3 x^{2} y + 3 y^{2} - 2 y)$	Coma y	0.034
$Z_{7}$	$8^{1 ∕ 2} (3 x^{3} + 3 {xy}^{2} - 2 x)$	Coma x	$- 0.051$
$Z_{8}$	$8^{1 ∕ 2} (3 x^{2} y - y^{3})$	Trefoil y	0.031
$Z_{9}$	$8^{1 ∕ 2} (x^{3} - 3 {xy}^{2})$	Trefoil x	0.018
$Z_{10}$	$5^{1 ∕ 2} (6 (x^{4} + 2 x^{2} y^{2} + y^{4} - x^{2} - y^{2}) + 1)$	$1^{ary}$ spherical	$- 0.573$

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