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Enhancing fluorescence detection with a photonic crystal structure in a total-internal-reflection configuration

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Abstract

In contrast to fluorescence enhancement of fluorophores embedded in a photonic crystal structure as previously reported [Appl. Phys. Lett. 75, 3605 (1999) ], in this Letter we demonstrate a unique approach to forming an open microcavity using a one-dimensional photonic crystal in a total-internal-reflection geometry. This configuration opens up the possibility for enhancing fluorescence imaging and biosensing. Time-resolved fluorescence detection of fluorophores immobilized on the open cavity has been carried out. Over 20-fold fluorescence enhancement was observed.

© 2008 Optical Society of America

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