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Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

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Abstract

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3±0.4ms were achieved with the single-layer microfluidic device.

© 2008 Optical Society of America

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