Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Not Accessible

Your library or personal account may give you access

Abstract

This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.

© 2008 Optical Society of America

Full Article  |  PDF Article
More Like This
Cell tracking and detection of molecular expression in live cells using lipid-enclosed CdSe quantum dots as contrast agents for epi-third harmonic generation microscopy

Chieh-Feng Chang, Chao-Yu Chen, Fu-Hsiung Chang, Shih-Peng Tai, Cheng-Ying Chen, Che-Hang Yu, Yi-Bing Tseng, Tsung-Han Tsai, I-Shuo Liu, Wei-Fang Su , and Chi-Kuang Sun
Opt. Express 16(13) 9534-9548 (2008)

In vivo and ex vivo imaging of intra-tissue elastic fibers using third-harmonic-generation microscopy

Che-Hang Yu, Shih-Peng Tai, Chun-Ta Kung, I-Jong Wang, Han-Chieh Yu, Hsiang-Ju Huang, Wen-Jeng Lee, Yi-Fan Chan, and Chi-Kuang Sun
Opt. Express 15(18) 11167-11177 (2007)

Higher harmonic generation microscopy of in vitro cultured mammal oocytes and embryos

Cho-Shuen Hsieh, Shee-Uan Chen, Yen-Wei Lee, Yu-Shih Yang, and Chi-Kuang Sun
Opt. Express 16(15) 11574-11588 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved