Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

Not Accessible

Your library or personal account may give you access

Abstract

We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5×-extended depth-of-field imaging of developing embryos.

© 2009 Optical Society of America

Full Article  |  PDF Article
More Like This
Multiscale Bessel beams generated by a tunable acoustic gradient index of refraction lens

Euan McLeod, Adam B. Hopkins, and Craig B. Arnold
Opt. Lett. 31(21) 3155-3157 (2006)

Resolution enhancement in an extended depth of field for volumetric two-photon microscopy

Hongsen He, Cihang Kong, Ka Yan Chan, W. L. So, Hiu Ka Fok, Yu-Xuan Ren, Cora S. W. Lai, Kevin K. Tsia, and Kenneth K. Y. Wong
Opt. Lett. 45(11) 3054-3057 (2020)

Extended depth of field microscopy for rapid volumetric two-photon imaging

Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy
Opt. Express 21(8) 10095-10104 (2013)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Supplementary Material (1)

Media 1: AVI (4311 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved