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Laboratory soft-x-ray microscope for cryotomography of biological specimens

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Abstract

Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for re searchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to 100nm.

© 2011 Optical Society of America

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Supplementary Material (2)

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Media 2: MOV (2632 KB)     

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