Abstract

We present an application of second-harmonic generation (SHG) microscopy for label-free visualization and quantification of the morphology of nerve fibers in the retina. We show that SHG arises from the retinal nerve fiber layer and that it is specifically associated with uniformly oriented microtubules in the axons. The utility of axonal SHG is demonstrated for imaging the neuroanatomy of fresh ex vivo retina, and the three-dimensional structure of the axons of retinal ganglion cells is quantitatively analyzed.

©2012 Optical Society of America

Label-free nonlinear optical imaging of mouse retina

Sicong He, Cong Ye, Qiqi Sun, Christopher K.S. Leung, and Jianan Y. Qu
Biomed. Opt. Express 6(3) 1055-1066 (2015)

Retinal nerve fiber layer reflectometry must consider directional reflectance

Xiang-Run Huang, Robert W. Knighton, William J. Feuer, and Jianzhong Qiao
Biomed. Opt. Express 7(1) 22-33 (2016)

Imaging axonal transport in the rat visual pathway

Carla J. Abbott, Tiffany E. Choe, Theresa A. Lusardi, Claude F. Burgoyne, Lin Wang, and Brad Fortune
Biomed. Opt. Express 4(2) 364-386 (2013)

Light scattering and form birefringence of parallel cylindrical arrays that represent cellular organelles of the retinal nerve fiber layer

Qienyuan Zhou and Robert W. Knighton
Appl. Opt. 36(10) 2273-2285 (1997)

Retinal nerve fiber bundle tracing and analysis in human eye by polarization sensitive OCT

Mitsuro Sugita, Michael Pircher, Stefan Zotter, Bernhard Baumann, Philipp Roberts, Tomoyuki Makihira, Nobuhiro Tomatsu, Makoto Sato, Clemens Vass, and Christoph K. Hitzenberger
Biomed. Opt. Express 6(3) 1030-1054 (2015)