Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Fluorescence holography with improved signal-to-noise ratio by near image plane recording

Not Accessible

Your library or personal account may give you access

Abstract

Holographic fluorescence imaging is very promising, as it can obtain three-dimensional fluorescence imaging without scanning. However, the current method usually records holograms far from the image plane, with the fluorescence decaying when spreading broadly. Here we show that the signal-to-noise ratio (SNR) of fluorescence holography can be improved by recording the high-contrast interferogram near the image plane. We found that this can be achieved by setting the focal length of the lens for the reference wave (f2) close to that for the object wave (f1). With experiments, we demonstrate an example of an increase of about 21 times in SNR by changing f2 from infinity to 226 mm, which is close to f1 (323 mm).

©2012 Optical Society of America

Full Article  |  PDF Article
More Like This
Reconstruction of objects above and below the objective focal plane with dimensional fidelity by FINCH fluorescence microscopy

Nisan Siegel, Joseph Rosen, and Gary Brooker
Opt. Express 20(18) 19822-19835 (2012)

Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope provides an improved Signal to Noise Ratio

Klaus Greger, Manuel J. Neetz, Emmanuel G. Reynaud, and Ernst H.K. Stelzer
Opt. Express 19(21) 20743-20750 (2011)

Self-interference compressive digital holography with improved axial resolution and signal-to-noise ratio

Tianlong Man, Yuhong Wan, Fan Wu, and Dayong Wang
Appl. Opt. 56(13) F91-F96 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved