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Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, and Jianan Y. Qu

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Abstract

We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).

©2012 Optical Society of America

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