Abstract

We present a hybrid microscope combining multiphoton microscopy incorporating second-harmonic generation contrast and optical-resolution optoacoustic (photoacoustic) microscopy. We study the relative performance of the two systems and investigate the complementarity of contrast by demonstrating the label-free imaging capabilities of the hybrid microscope on zebrafish larvae ex vivo, concurrently visualizing the fish musculature and melanocytes. This implementation can prove useful in multiparametric microscopy studies, enabling broader information to be collected from biological specimens.

© 2014 Optical Society of America

All-optical optoacoustic microscope based on wideband pulse interferometry

Georg Wissmeyer, Dominik Soliman, Rami Shnaiderman, Amir Rosenthal, and Vasilis Ntziachristos
Opt. Lett. 41(9) 1953-1956 (2016)

Fiber interferometer for hybrid optical and optoacoustic intravital microscopy

Rami Shnaiderman, Georg Wissmeyer, Markus Seeger, Dominik Soliman, Hector Estrada, Daniel Razansky, Amir Rosenthal, and Vasilis Ntziachristos
Optica 4(10) 1180-1187 (2017)

Time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo

Simon P. Poland, Nikola Krstajić, Simao Coelho, David Tyndall, Richard J. Walker, Viviane Devauges, Penny E. Morton, Nicole S. Nicholas, Justin Richardson, David Day-Uei Li, Klaus Suhling, Claire M. Wells, Maddy Parsons, Robert K. Henderson, and Simon M. Ameer-Beg
Opt. Lett. 39(20) 6013-6016 (2014)

Ultrawideband reflection-mode optoacoustic mesoscopy

Murad Omar, Dominik Soliman, Jérôme Gateau, and Vasilis Ntziachristos
Opt. Lett. 39(13) 3911-3914 (2014)

Hybrid optical and acoustic resolution optoacoustic endoscopy

Hailong He, Georg Wissmeyer, Saak V. Ovsepian, Andreas Buehler, and Vasilis Ntziachristos
Opt. Lett. 41(12) 2708-2710 (2016)